pf 127 solutions  (MedChemExpress)

Journal: Journal of Cellular and Molecular Medicine

Article Title: Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing

doi: 10.1111/jcmm.18130

Figure Lengend Snippet: The construction and characterization of PF‐127@P dressings. (A) Image of PF‐127@P hydrogels at 4°C and 37°C. (B) Cumulative release rate (%) of PF‐127@P hydrogels containing different concentrations of pectolinarin at 1, 2, 3, 6, 12, 24 and 36 h in vitro.

Article Snippet: PF‐127 solutions containing pectolinarin (MedChemExpress, China) at concentrations of 0, 25, 50, 100 and 200 μg/mL were prepared in an ice bath, and the gel performance was evaluated.

Techniques: In Vitro

Journal: Journal of Cellular and Molecular Medicine

Article Title: Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing

doi: 10.1111/jcmm.18130

Figure Lengend Snippet: Biocompatibility of PF‐127@P dressings. (A) Live/dead staining of coculture with dressing's extract for 1, 2 and 3 days, red is dead cells and green is live cells. (B) Hemolysis rate. * p < 0.05 when compared with PF‐127. (C) Cytoskeleton staining of coculture with dressing's extract for 1, 2 and 3 days, blue is nucleus and green is cytoskeleton. (D) Changes in body weight of experimental group (red, hydrogel wiping group) and blank group (black). (E) Major organ changes in rats with dressing (experimental) and control group.

Article Snippet: PF‐127 solutions containing pectolinarin (MedChemExpress, China) at concentrations of 0, 25, 50, 100 and 200 μg/mL were prepared in an ice bath, and the gel performance was evaluated.

Techniques: Staining, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing

doi: 10.1111/jcmm.18130

Figure Lengend Snippet: Dynamic process of wound healing under PF‐127@P dressings. (A) The dynamic process and schematic diagram of skin defect repair in rats with PF‐127@P hydrogels at Days 0, 3, 7, 10 and 14. (B–E) Wound healing rates at days 3, 7, 10 and 14. * p < 0.05 when compared with control.

Article Snippet: PF‐127 solutions containing pectolinarin (MedChemExpress, China) at concentrations of 0, 25, 50, 100 and 200 μg/mL were prepared in an ice bath, and the gel performance was evaluated.

Techniques: Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing

doi: 10.1111/jcmm.18130

Figure Lengend Snippet: Wound healing image and the scarless regeneration mechanism. (A) Haematoxylin and eosin and Masson staining images of wound on day 14 under PF‐127@P dressing. (B) The results of molecular docking experiments of the action of pectolinarin and YAP. (Yellow is the hydrogen bond, red is the site of pectolinarin binding on YAP and green is pectolinarin.) (C) Expression of α‐SMA; YAP and En1. α‐SMA, α‐smooth muscle Actin; En1, Engrailed‐1; YAP, Yes1 associated transcriptional regulator. * p < 0.05 when compared with control.

Article Snippet: PF‐127 solutions containing pectolinarin (MedChemExpress, China) at concentrations of 0, 25, 50, 100 and 200 μg/mL were prepared in an ice bath, and the gel performance was evaluated.

Techniques: Staining, Binding Assay, Expressing, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing

doi: 10.1111/jcmm.18130

Figure Lengend Snippet: Immunohistochemistry (IHC) image of wound healing. (A) Expression of CD34 (yellow arrows represented CD34) in the Control and PF‐127@50 groups on days 7 and 14. (B) Immunohistochemical statistics of vessel number in the Control and PF‐127@50 groups on days 7 and 14. (C) Statistical plot of YAP expression in tissues at day 14. (D) Statistical plot of YAP expression in tissues at day 14. (E) Expression of YAP in tissues at day 14. (F) Expression of α‐SMA in tissues at day 14. * p < 0.05 when compared with control.

Article Snippet: PF‐127 solutions containing pectolinarin (MedChemExpress, China) at concentrations of 0, 25, 50, 100 and 200 μg/mL were prepared in an ice bath, and the gel performance was evaluated.

Techniques: Immunohistochemistry, Expressing, Control, Immunohistochemical staining

Journal: bioRxiv

Article Title: A high-throughput nematode sensory assay reveals an inhibitory effect of ivermectin on parasite gustation

doi: 10.1101/2023.04.25.538347

Figure Lengend Snippet: A schematic overview of the procedures used to produce the data in B-D. C. elegans are washed from NGM culture plates into a 1.5 mL tube and washed 3 times to remove residual debris and bacteria and to enrich for young adult worms. Worms are then mixed with liquid PF-127 and quickly added to the devices to allow for polymerization of the hydrogel. Initial experiments used 24-well devices with 3 slices, and later experiments used the gustatory microplate. Cues and controls are added as liquid bubbles to the top and bottom of the polymer, and then devices are incubated in a humid chamber to allow for worm gustation and burrowing. (B) C. elegans are attracted to either 100 mM or 200 mM NaCl, with a slightly higher CI when the cue was added to the top of the well. (C) Three-layer Stacks devices were separated after 1 hr. incubation, and worms in each slice were counted. Wild type (N2) worms are attracted to 200 mM NaCl and repelled by 10 mM quinine. tax-4(p678) worms show no response to NaCl and are attracted to quinine. (D) Chemotaxis indices of five chemosensory-defective knockout strains of C. elegans in response to 200 mM NaCl and 10 mM quinine. In each figure, dots represent the value for a single well of the device, large red dots represent the mean, and error bars represent the confidence limits. ***: p <= 0.001, ****: p <= 0.0001.

Article Snippet: In addition to this, a 30% w/w PF-127 (Sigma Aldrich)/water gel solution was created 2-3 days before assay date to allow the PF-127 to dissolve completely at 4°C.

Techniques: Incubation, Chemotaxis Assay, Knock-Out

Journal: bioRxiv

Article Title: A high-throughput nematode sensory assay reveals an inhibitory effect of ivermectin on parasite gustation

doi: 10.1101/2023.04.25.538347

Figure Lengend Snippet: (A) A schematic overview of the procedures used to produce the data in B. L3s were extracted from mosquitoes and washed 3 times prior to being mixed with liquid PF-127 and added to devices to allow for polymerization. Cues and controls are added as liquid bubbles to the top and bottom of the polymer, and devices are incubated in a humid chamber to allow for worm gustation and burrowing. (B) L3s from Brugia pahangi are attracted to FBS. Two methods for calculating chemotaxis index are shown. Dots represent the CI for a single well of the device, large red dots represent the mean, and error bars represent the confidence limits. *: p <= 0.05.

Article Snippet: In addition to this, a 30% w/w PF-127 (Sigma Aldrich)/water gel solution was created 2-3 days before assay date to allow the PF-127 to dissolve completely at 4°C.

Techniques: Incubation, Chemotaxis Assay